Chromatin tracing and Multiplexed Imaging of Nucleome Architectures (MINA) in single mammalian cells and tissue

. Author manuscript; available in PMC: 2022 Apr 13.

Published in final edited form as: Nat Protoc. 2021 Apr 26;16(5):2667–2697. doi: 10.1038/s41596-021-00518-0

Solution	Flow speed (mL/min)	Volume (mL)
Secondary probes	0.6	2.5
Secondary probe hybridization	0	Hybridize for 30 min.
Secondary wash buffer	0.6	1.2
Imaging buffer	0.6	1.2