Fig. 3. Nsp1-deficient replicons are hypersensitive to interferons.

(A and B) Time course measurements of Gluc in the supernatant (A) or cell viability (B) of Huh-7.5 cells electroporated with WT or Nsp1 K164A/H165A double mutant (Nsp1^mut) Gluc replicon RNA. Cells were seeded with 100 nM remdesivir or vehicle and were washed in phosphate-buffered saline 24 hours before each respective time-point collection. Mock-electroporated cells were used as controls for post-electroporation cell viability. The dashed line indicates the limit of detection. N = 4. Error bars indicate SD. (C to E) Huh-7.5 cells were electroporated with WT or Nsp1^mut replicon RNA and seeded on 96-well plates containing the indicated concentrations of (C) IFNα, (D) IFNβ, or (E) remdesivir. Gluc activity (filled circles) and cell viability (empty circles) were measured 24 hours after electroporation. N = 4. Error bars indicate SD.