TABLE 1.
HPLC validation
| Naringenin | 8‐PN | |
|---|---|---|
| Recovery a (%) | ||
| Kidney | 69 ± 11 | 110 ± 17 |
| Liver | 95 ± 19 | 123 ± 29 |
| Muscle | — | 118 ± 17 |
| Lung | — | 80 ± 703 |
| Pancreas | — | 93 ± 7.8 |
| Heart | — | 112 ± 0.8 |
| Spleen | — | 101 ± 17 |
| Brain | — | 59 ± 54 |
| Intra‐day CV b % | 2.5 | 3.6 |
| Inter‐day CV b % | 6.9 | 2.4 |
—, not analyzed.
a
Values are presented as the means ± SD (n = 3). 8‐PN or naringenin (100 pmol) was added to each tissue homogenate and extracted based on the sample preparation method.
b
These CV were determined by comparing different injections on the same day (n = 3, the intra‐day) or different injections on different days (n = 3, the inter‐day).