(A) DISPA-PRM quantitative outputs for six target peptides analyzed during a one-minute acquisition. (B) Further evaluation of the proxy ISPG peptide, ETDIGVTGGGQGK, reveals the top ten fragment ions and their intensities across the one-minute analysis. Of the ten most intense fragment ions, the transitions y7+, y8+, and y9+ were used for quantitation of the peptide.