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. 2022 Apr 13;13(4):345. doi: 10.1038/s41419-022-04809-6

Fig. 2. Resolving neutrophils trained by 4-PBA express elevated levels of cell surface CD86/CD200R, increased RvD1/SerpinB1, and reduced TNF-α.

Fig. 2

A Flow analyses of mature Ly6GhiCD11b+ neutrophils. WT neutrophils treated with PBS or 4-PBA (1 mM) for 24 hours were subjected to flow analyses and the percentages of Ly6GhiCD11b+ neutrophils were plotted. (n = 3). B The mean fluorescent intensity (MFI) levels of Ly6G on WT neutrophils treated with PBS or 4-PBA (1 mM) for 24 hours (n = 3). C The levels of CD86 and CD200R on WT neutrophils treated with PBS or 4-PBA (1 mM) for 24 hours (n = 3). D The expression of RvD1 and SerpinB1 from WT neutrophils treated with PBS or 4-PBA (1 mM) for 24 hours (n = 3). E The secretion of TNF-α from WT neutrophils treated with PBS or 4-PBA (1 mM) for 24 hours followed by PMA priming (100 ng/ml) for 30 min (n = 3). Data plotted as mean ± SD. ****P < 0.0001, ***P < 0.001, **P < 0.01 using two-sided Student’s t test.