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. 2022 Mar 9;11:e76963. doi: 10.7554/eLife.76963

Figure 1. Osteoblast proteins are enriched with the amino acid proline.

(A) Heat map depicting the relative amino acid enrichment for the indicated osteoblast proteins. Color bar represents fold enrichment relative to the average amino acid content. White boxes denote below-average enrichment. (B) Heat map depicting alanine or proline enrichment in differentiation-associated proteins. Color bar represents the percent increase in abundance relative to all proteins. (C) Volcano plot depicting the proline proportion of the top 500 genes that are induced or suppressed during osteoblast differentiation. Dashed lines denote quartiles while the solid line denotes the median. ****p≤0.00005 by unpaired two-tailed Student’s t-test. (D) Graphical depiction of the predicted change in demand for alanine or proline based on changes in gene expression during osteoblast differentiation. See numerical source data and RNAseq source data in Figure 1—source data 1.

Figure 1—source data 1. Numerical source data for Figure 1.

Figure 1.

Figure 1—figure supplement 1. Predicted amino acid demand changes during osteoblast differentiation.

Figure 1—figure supplement 1.

(A) Functional assays of calvarial cells cultured in growth media (GM) or osteogenic medium (OM) for 10 days (n = 3). (B) qRT-PCR analysis of osteogenic marker genes Runx2, Sp7 (OSX), Col1a1, and Bglap in calvarial cells cultured in GM or OM for 7 days (n = 3). Error bars depict SD. (C) Graphical depiction of the predicted change in demand for cysteine based on changes in gene expression during osteoblast differentiation. See numerical source data in Figure 1—figure supplement 1—source data 1.
Figure 1—figure supplement 1—source data 1. Numerical source data for Figure 1—figure supplement 1.