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. Author manuscript; available in PMC: 2022 Apr 13.
Published in final edited form as: Mol Microbiol. 2020 Nov 3;115(2):272–289. doi: 10.1111/mmi.14615

FIGURE 6.

FIGURE 6

Stressed Mtb cells lacking clpB are hypersensitive to GA-mediated inhibition of HtpG. (a) Schematic of experimental set-up for evaluating chemical inhibition of HtpG by GA with and without nonessential chaperones under replicating (nutrient-rich) and nonreplicating (host stress model with nitrite) conditions as shown in Figure 4c. (b) CFU analysis demonstrates that survival is significantly reduced under nonreplicating conditions for all strains tested, but there is no change under replicating conditions (Figure S21). Compared to Figure 4c, these results suggest that GA addition phenocopies genetic depletion experiments, but might exhibit off-target effects or enhance cell death through different modes of action. *p < .0332, **p < .0021, ***p < .0002, ****p < .0001, two-way ANOVA or student’s t test was used for comparison (n = 3, error bars represent SD)