Fig. 3.
Semiquantitative gene expression level of GABAAρ1 and histamine H3 receptors for fold increase, the relative level of GABA and histamine gene expression is normalized to GAPDH. The result was calculated with software from Alpha view SA (version 3.3.1, Cell Biosciences Inc., Victoria, VIC, Australia). C1, negative control (cell control); C2, positive control GABA (1 mg/mL); S1, ashwagandha extract at 15 μg/mL; S2, ashwagan-dha extract at 30 μg/mL; GABA, γ-aminobutyric acid; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.