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. Author manuscript; available in PMC: 2022 Sep 2.
Published in final edited form as: Nat Struct Mol Biol. 2022 Mar 2;29(3):229–238. doi: 10.1038/s41594-022-00735-5

Figure 1. SARS-CoV-2 spike ectodomain and Hydrogen-Deuterium Exchange monitored by mass spectrometry (HDX-MS) experimental overview.

Figure 1.

(A) Schematic of the prefusion-stabilized SARS-CoV-2 spike protein and a model of the trimeric prefusion conformation (24). (B) Schematic of HDX-MS experiment and the resulting mass distributions for a peptide that exists in either one (left) or two (right) separable conformations. In order for the two conformations to result in a bimodal mass distribution, they must not interconvert during the timescale of the HDX experiment (hours). Rapid interconversion would result in a single mass distribution with the ensemble averaged mass profile.