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. 2022 Apr 11;221(6):e202103069. doi: 10.1083/jcb.202103069

Figure 2.

Figure 2.

Apical accumulation of Rab11 vesicles depends on apical Myosin II activation. (A) Inhibition of Myosin II activation by Y-27632 injection at late cellularization prevents apical Rab11 vesicle accumulation. Zoom-in view of the outlined region in A. 0 s: onset of gastrulation. Y-27632 injection: N = 4 embryos; H2O injection: N = 3 embryos. (B) Injection of Y-27632 during apical constriction causes rapid diminishing of apical Rab11 vesicles. N = 4 embryos. (B’) Cross-section view. Yellow lines: apical surface. (B’’) Zoom-in view of B. (C) Knockdown of myosin heavy chain Zipper inhibits apical accumulation of Rab11 vesicle. N = 4 embryos for each genotype. (C’) Zoom-in view of C. (D) Spn27A/Df mutant embryos exhibit ventralized phenotype with expanded domain of apical Myosin II activation and apical Rab11 vesicle accumulation (N = 3 embryos). (D’) Zoom-in view of D. Images were Gaussian filtered with a radius of 0.5 pixel. Fewer z-slices were projected for the zoom-in view to improve the signal/noise. All scale bars for zoom-in view, 2 μm; scale bars for the rest, 10 μm. All 10 face views are maximum projections of a ∼3–4 μm-stack below apical surface of the constriction domain.