Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 11;221(6):e202103069. doi: 10.1083/jcb.202103069

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Chen and He

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Apical actomyosin activation, but not apical cell area reduction, is critical for apical accumulation of Rab11 vesicles. (A) Ectopic expression of Fog results in Myosin II activation in dorsal cells but does not cause apical area reduction. N = 3 embryos. Scale bar, 10 μm. (B and C) Ectopic activation of Myosin II in dorsal cells by overexpression of Fog induces ectopic apical accumulation of Rab11 vesicles. (B) Enface view. (C) Cross-section view. N = 5 embryos for Fog overexpression; N = 4 embryos for control embryos. Scale bars, 5 μm. Scale bar for zoom-in view, 2 μm. (D and E) Comparison of YFP-Rab11 in the ventral furrow and dorsal fold. Yellow outlines: example cells displaying apical area reduction. Dorsal fold cells do not show apical Rab11 vesicle accumulation despite undergoing apical shrinking. N = 10 embryos for imaging the dorsal folds. (D and E) Cross-section view and en face view, respectively. Scale bar, 5 μm. Green arrows: Rab11 vesicles. Scale bar, 5 μm.