TABLE 2.

Effect of DOX and human serum on viability and sterol composition of ATCC 2001 and 97SQS cells^a

Strain	Medium supplementation		Viability (log CFU/ml)	Sterol content (μg/g [dry/wt] of cellsb
	DOX (μg/ml)	Human serum (%)		Cholesterol	Ergosterol	Lanosterol	Total
ATCC 2001	0	0	9.00 ± 0.04	0d	276.0 ± 3.0	4.75 ± 0.6	280.7 ± 2.4
	10	0	NAc	0d	287.6 ± 17.4	5.9 ± 0.4	293.4 ± 17.6
	0	10	8.89 ± 0.03	975.4 ± 50.1	218.4 ± 6.9	8.1 ± 1.0	1,201.85 ± 44.2
97SQS	0	0	8.77 ± 0.04	0d	378.4 ± 20.3	9.5 ± 0.5	387.8 ± 20.8
	10	0	6.67 ± 0.03	0d	70.7 ± 8.7	UDe	70.7 ± 8.7
	0	10	8.82 ± 0.04	409.6 ± 1.7	239.9 ± 29.5	4.8 ± 3.3	654.3 ± 26.3
	10	10	8.33 ± 0.07	667.3 ± 22.9	92.9 ± 9.6	0.3 ± 0.2	667.3 ± 22.9

^aValues are means ± standard deviations (three independent samples per group). 

^b4,4-Diphenyl-1-benzyl-piperidine was used as the internal standard in these experiments. 

^cNA, not assayed. 

^dNo cholesterol detected. 

^eUD, under the detection limit.