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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Trends Genet. 2021 Dec 18;38(5):437–453. doi: 10.1016/j.tig.2021.11.006

Figure 1. In vivo methods for introducing CRISPR reagents into Drosophila.

Figure 1.

A. Plasmids encoding sgRNA and Cas9 are injected into Drosophila embryos to induce DSB in the germline. Donors can be added as circular plamsids, in vivo linearized dsDNA or ssDNA. B. Recombinant Cas enzymes and chemically synthesized gRNA are combined into a Ribonucleoprotein (RNP) for injection. C. Plasmid encoding sgRNA is injected into embryos from flies expressing Cas9 from a germline promoter. D. Transgenic sgRNA-expressing flies are crossed to transgenic germline-expressing Cas9 flies to produce germline edits. E. Transgenic sgRNA-expressing flies are crossed to transgenic tissue-specific Cas9 flies to produce somatic tissue edits.