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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Trends Genet. 2021 Dec 18;38(5):437–453. doi: 10.1016/j.tig.2021.11.006

Table 1:

Drosophila focused CRISPR resources

Resource Type Comments Refs Webpage
DRSC Find CRISPR Tool guide RNA design Fly sgRNA designs with genome view [78] https://www.flyrnai.org/crispr3/web/
SNP-CRISPR guide RNA design Accepts variant annotations as the input [95] https://www.flyrnai.org/tools/snp_crispr/web/
CRISPR Optimal Target Finder guide RNA design Includes multiple Drosophila and insect species [46] http://targetfinder.flycrispr.neuro.brown.edu
TRiP-CRISPR transgenic CRISPR fly stocks and plasmids Search for and nominate CRISPR-a and CRISPR-KO fly stocks [15] https://www.flyrnai.org/tools/grna_tracker/web/
CRISPR Fly Design transgenic CRISPR fly stocks and plasmids CRISPR stocks and protocols for genome engineering https://www.crisprflydesign.org/
FlyCRISPR transgenic CRISPR fly stocks and plasmids CRISPR stocks and protocols for genome engineering https://flycrispr.org
FlyCas9 transgenic CRISPR fly stocks and plasmids CRISPR stocks and protocols for genome engineering https://shigen.nig.ac.jp/fly/nigfly/cas9/
DRSC/TRiP cell based and transgenic CRISPR reagents Includes online tools, protocols, and large scale resources https://fgr.hms.harvard.edu/fly-cellcrispr-cas
DGRC cells lines and plasmids Collects and distributes CRISPR plasmids and cell lines https://dgrc.bio.indiana.edu/Home
BDSC transgenic CRISPR fly stocks Collects and distributes transgenic CRISPR fly stocks https://bdsc.indiana.edu
VDRC transgenic CRISPR fly stocks Collects and distributes transgenic CRISPR fly stocks https://stockcenter.vdrc.at/control/main