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. Author manuscript; available in PMC: 2023 Apr 12.
Published in final edited form as: Circulation. 2022 Mar 17;145(15):1140–1153. doi: 10.1161/CIRCULATIONAHA.121.056929

Figure 5. MitoQ rescues the effect of miR-210 deficiency on mitochondrial bioenergetics.

Figure 5.

Male miR-210 knockout (KO) and their wild-type (WT) littermate control mice with C57Bl/6 background at 2 months old underwent myocardial infarction (MI) induced by in vivo heart ischemia and reperfusion (IR) treatment via ligation of the mid-left anterior descending coronary artery for 30 min followed by reperfusion. MitoQ (4 mg/kg) or vehicle control (DMSO, 1%) was intravenously applied 15 min prior to IR. The hearts were harvested 24 h after IR and mitochondrial oxygen consumption rate (OCR) (A-F) and extracellular acidification rate (ECAR) (G-I) were analyzed by Seahorse XFe24 Analyzer in isolated cardiac muscle fiber bundles. Seahorse real-time traces and averaged data for OCR (A) and ECAR (G) including the chemical agents that dissect the metabolic responses are shown. Data are means ± SD, with n=5 animals per group, and analyzed by two-way ANOVA followed by Tukey’s test. P values are shown in the Figure.