A. Focused re-clustering 2,798 ciliated cells of healthy fallopian tubes identifies 4 subtypes, 1–1 to 1–4, shown in UMAP space.
B-E. Expression levels of selected markers used to identify the 4 ciliated cell subtypes, with general markers for ciliated cells shown in B; markers for 1–1 and 1–2 in C; those for 1–3 in D; and those for 1–4 in E.
F. Immunofluorescence (IF) staining of the fallopian tube epithelium using antibodies against FOXJ1 and unique markers for ciliated cell subtypes: CDKN2A for 1–2, PAX8 for 1–3, LGALS1 and ACTA2 for 1–4. Arrows indicate double positive cells.
See also Figure S2, Figure S4, Table S4 and Table S6.