Fig. 1.

Neutralization of SARS-CoV-2 Lambda variant by convalescent and inactivated vaccine-elicited plasma. (a) Mutations located in the spike protein were identified in Lambda variant compared with the wild-type (WT). (b) The location of L452 and F490 residuals (red) in the ACE2 (yellow) and SARS-CoV-2 RBD (black) complex (PDB: 7DMU). (c, d) Changes in neutralizing titers of convalescent (c) and inactivated vaccine-elicited plasma (d) against Lambda variant, L452Q/F490S, L452Q, and F490S mutated viruses compared with those against the D614G-WT. The data shown here are means of two independent experiments. Geometric mean titers (GMTs) in ID50 values and fold-change were calculated and shown above each variant in the left panel. The fold change in ID50 of each plasma against mutated viruses were represented in the right panel. Statistical analysis was performed with two-side Friedman test with Dunn's multiple comparison using GraphPad Prism 9 software. ns: P > 0.05; ***: P < 0.001, ****: P < 0.0001. The changes between 3-fold and 5-fold are marked in blue. The changes between 5-fold and 10-fold are marked in orange, and those above 10-fold are marked in red. The symbol ‘+’ indicates increased neutralization, and ‘-’ indicates decreased neutralization.