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. 2022 Apr 13;12:6154. doi: 10.1038/s41598-022-09964-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PD-L1xCD3 BiTE constructs and expression in HEK293T. (A) PD-L1xCD3 BiTE gene arrangement of mBiTE and sBiTE (B) Construct map of PD-L1xCD3 BiTE in pCDH plasmid backbone; RFP (red fluorescent protein), T2A (self-cleaving peptide), IRES (internal ribosome entry site), and PuroR (Puromycin resistant gene). PD-L1xCD3 BiTE protein produced from HEK293T was detected by Coomassie Brilliant Blue staining (C) and Western blot analysis (D).