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. 2022 Apr 13;12:6154. doi: 10.1038/s41598-022-09964-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of BiTEs on the induction of T cell cytotoxicity against CCA cells. A killing assay was performed to determine the number of remaining mCherry-expressing CCA cells after coculturing of T cells in the presence or absence of mBiTE or sBiTE. The remaining cells were observed after 24 h of coculturing under the florescence microscope at an E:T ratio of 10:1 (left). The bar graph showed the percentage of cell death relative to non-treatment control at E:T ratios of 0:1, 2.5:1, 5:1, and 10:1 (right). Data are presented as the mean ± standard error of the mean (SEM) (error bar) (*p < 0.05; **p < 0.01; ***p < 0.001).