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. 2022 Apr 13;13(4):340. doi: 10.1038/s41419-022-04803-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Four groups were created. IGFBP5-OE cells and control HUVECs were cultured under NG conditions. HUVECs were cultured in HG conditions and transfected with or without siIGFBP5. The levels of lactic acid (A), pyruvate (B), and F-2,6-BP (C) were measured in each group. n = 3. The levels of lactic acid (D), pyruvate (E), and F-2,6-BP (F) were measured in control and IGFBP5-OE cells that were transfected with or without siPFKFB3 under NG conditions. n = 3. G Glycolytic flux analysis of NG, NG + siPFKFB3, NG + siIGFBP5, and NG + IGFBP5-OE cells by a Seahorse Flux Analyzer. The extracellular acidification rate (ECAR) was recorded after the injection of glucose, oligomycin, and 2-deoxyglucose (2-DG). H–J Statistical analysis of glycolysis, the glycolytic capacity, and glycolytic reserves in the ECAR. K Glycolytic flux analysis of NG, HG, HG + siPFKFB3, and HG + siIGFBP5 HUVECs by a Seahorse Flux Analyzer. The ECAR was recorded after the injection of glucose, oligomycin, and 2-DG. L–N Statistical analyses of glycolysis, the glycolytic capacity, and glycolytic reserves in the ECAR. The data are shown as the mean ± SD in all statistical graphs. *P < 0.05, **P < 0.01, ***P < 0.001; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001.