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. 2022 Apr 13;13(4):342. doi: 10.1038/s41419-022-04807-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Flowchart illustrating the transcriptome analysis of paired primary and metastatic tissues of HGSOC (HGSOC 1-4 and HGSOC-M 1-4). B Clustered heatmap of differentially expressed mRNA in paired primary and metastatic tissues of four HGSOC patients. Red, up-regulation; Blue, down-regulation. C Venn diagrams showing the overlap of all differentially expressed mRNAs and eccDNAs of the same host gene. Left, up-regulated targets in both sequencing; Right, down-regulated targets in both sequencing. D Sanger sequencing results of PCR products of 15 bases on either side of junctions of eccDNAs derived from TIAM1, PKNOX1, DNMT1, ABI3BP and RORA,were listed in boxes, respectively. Black (upper) indicated the junction sequences of each eccDNA by Circle-Seq, with shaded (red) sequences depicting junction sites. Blue (lower) indicated the accurate circularization sites of each eccDNA based on Sanger sequencing results. E qPCR validations of 5 differentially expressed mRNAs (upper panel), and differentially expressed eccDNAs (lower panel) in 20 clinical HGSOC tissue samples containing 20 primary tissue samples (blue, HGSOC) and paired metastatic tissue samples (yellow, HGSOC-M). The data of the expression levels were shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.