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. 2022 Apr 13;13:1989. doi: 10.1038/s41467-022-29633-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative images of the Chd7^fl/fl and Prx1-cre;Chd7^fl/fl mice at postnatal day 0, day 7, and day 28. b Alizarin red/Alcian blue whole-mount skeletal staining images of the Chd7^fl/fl, Prx1-cre;Chd7^fl/+, and Prx1-cre;Chd7^fl/fl pups at postnatal day 2. Compared to the control littermates, The Prx1-cre;Chd7^fl/fl pups had shorter forelimbs and hindlimbs and a lower degree of mineralization in the cranial and maxillofacial bone. Scale bar, 5 mm. c Quantification of (b), including the length of humeri, femurs, and tibiae of the mice at postnatal day 2 (n = 8). d Representative reconstructed microCT images of skulls of the 2-week-old and 4-week-old Chd7^fl/fl and Prx1-cre;Chd7^fl/fl mice. The skulls of Prx1-cre;Chd7^fl/fl mice showed nonclosure of fontanels. Scale bar, 2 mm. e Quantification of (d), cranial defect area calculated by ImageJ (n = 8). Data are shown as the mean ± S.D.; p value by two-tailed for independent sample tests or one-way ANOVA with Tukey’s post hoc tests for multiple comparisons.