TABLE 1.
Effect of lipid composition on (RFB/L)f and IE
| Liposome type, effect, and lipid composition | (RFB/L)f (nmol/μmol)a | IE (%)a | Lipid molar ratio |
|---|---|---|---|
| Nonsized liposomesb | |||
| Effect of Chol | |||
| PG (−6°C)c | 99 ± 1 | 98 ± 1 | |
| PG-Chol | 87 ± 2 | 79 ± 2 | 7:1 |
| DMPC (+20°C) | 60 ± 3 | 48 ± 2 | |
| DMPC-Chol | 40 ± 2 | 35 ± 3 | 7:1 |
| PC (−6°C) | 45 ± 4 | 51 ± 3 | |
| PC-Chol | 31 ± 2 | 33 ± 2 | 7:1 |
| Fluidity of membrane | |||
| PC-PG (−6 and −6°C) | 97 ± 3 | 99 ± 1 | 9:1 |
| DOPC-DPPG (+20 and +42°C) | 77 ± 4 | 54 ± 3 | 9:1 |
| DPPC-DPPG (+42 and +42°C) | 57 ± 3 | 42 ± 2 | 9:1 |
| Sized liposomesd | |||
| PC-PI | 49 ± 7 | 61 ± 2 | 7:3 |
| 40 ± 2 | 43 ± 3 | 9:1 | |
| PC-PG | 53 ± 6 | 53 ± 2 | 7:3 |
| 39 ± 6 | 45 ± 5 | 9:1 | |
| PC-PS | 45 ± 1 | 53 ± 2 | 7:3 |
| 42 ± 1 | 50 ± 7 | 9:1 |
a
Values are means ± standard deviations for at least three independent preparations.
b
Initial lipid concentration, 16 μmol/ml; initial RFB concentration, 1.6 μmol/ml; mean liposome diameter, 0.6 to 0.8 μm;
c
Values in parentheses are Tcs and were obtained from another publication (17).
d
Effect of lipid charge. Initial RFB concentration, 1.6 μmol/ml; initial lipid concentration, 16 μmol/ml; liposome mean diameter, 0.3 μm.