Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 13;13(4):344. doi: 10.1038/s41419-022-04699-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A Venn diagram of upregulated genes from the GEPIA database, the top 500 survival-related genes, upregulated lncRNAs from the GSE100736 data set, and human lncRNA names from the GENCODE database. B A box plot of HOTAIR expression in TCGA-GBM. The red box on the left indicates GBM samples, and the gray box on the right indicates normal samples. *p < 0.05. C Correlation analysis of HOTAIR expression with the overall survival of GBM patients in TCGA-GBM. D RT-qPCR detection of HOTAIR expression in normal brain tissues (n = 40) and GBM tissues (n = 40). ****p < 0.0001. E RT-qPCR detection of HOTAIR expression in GBM cell lines and human primary astrocytes NHA. ****p < 0.0001, ns, not significant, compared with NHA cells. F Morphology of serum-EVs of GBM patients (n = 40) and healthy controls (n = 40) observed under a TEM. G Graph (left panel) and quantification (right panel) of NTA results of the size distribution and relative concentration of serum-EVs of GBM patients (n = 40) and healthy controls (n = 40), ****p < 0.0001. H Western blot analysis of CD63, CD9, TSG101, and GM130 proteins in the serum-EVs of GBM patients (n = 40) and healthy controls (n = 40). I RT-qPCR detection of HOTAIR expression in serum-EVs of GBM patients (n = 40) and healthy controls (n = 40). ****p < 0.0001. Each experiment was repeated three times independently.