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. 2022 Apr 13;13(4):344. doi: 10.1038/s41419-022-04699-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A miR-526b-3p expression detected by RT-qPCR and EVA1 mRNA and positive expression detected by RT-qPCR and immunohistochemistry in clinical tissues of GBM patients (n = 40) and normal brain tissues (n = 40). *p < 0.05, compared with normal brain tissues. B miR-526b-3p expression detected by RT-qPCR and EVA1 mRNA and protein expression detected by RT-qPCR and western blot analysis in U251 and LN229 cells treated with sh-HOTAIR or combined with oe-EVA1. C Invasion of U251 and LN229 cells treated with sh-HOTAIR or combined with oe-EVA1 measured by Transwell assay. D Colony formation of U251 and LN229 cells treated with sh-HOTAIR or combined with oe-EVA1 measured by colony formation assay. E RT-qPCR detection of miR-526b-3p and EVA1 expression in U251/TR and LN229/TR cells as well as parental cells U251 and LN229. F RT-qPCR detection of miR-526b-3p and EVA1 expression in U251/TR and LN229/TR cells treated with sh-HOTAIR or combined with oe-EVA1. G Colony formation of U251/TR and LN229/TR cells treated with sh-HOTAIR or combined with oe-EVA1 measured by colony formation assay. H Flow cytometric analysis of U251/TR and LN229/TR cell apoptosis upon treatment with sh-HOTAIR or combined with oe-EVA1. *p < 0.05, **p < 0.01, ***p < 0.001, or ****p < 0.0001. ns indicates no significant difference between the two groups. Each experiment was repeated three times independently.