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. 2022 Apr 13;13(4):344. doi: 10.1038/s41419-022-04699-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Tumor volume of mice injected with U251 cells co-cultured with GBM-serum-EVs or combined with Ad-sh-EVA1. B In vivo luminescence changes of mice injected with U251-LUC cells co-cultured with GBM-serum-EVs or combined with Ad-sh-EVA1. C Representative images showing xenografts and tumor weight analysis of mice injected with U251 cells co-cultured with GBM-serum-EVs or combined with Ad-sh-EVA1. D Immunohistochemistry analysis of Ki67 protein in tumor tissues of mice injected with U251 cells co-cultured with GBM-serum-EVs or combined with Ad-sh-EVA1. E RT-qPCR detection of HOTAIR, miR-526b-3p, and EVA1 expression in tumor tissues of mice injected with U251 cells co-cultured with GBM-serum-EVs or combined with Ad-sh-EVA1. *p < 0.05, **p < 0.01, ***p < 0.001 or ****p < 0.0001. ns indicates no significant difference between the two groups. Each experiment was repeated three times independently. n = 10 for mice following each treatment.