Fig. 1. CCR2 and CCR6 are specifically depleted from T lymphocytes in PLWH_EC.

Receptor expression on CD4⁺ and CD8⁺ T lymphocytes from PLWH_EC (n = 14), PLWH_ART (n = 54) and HC (n = 18). a Frequencies of CD4⁺ T cells and CD8⁺ T cells in total peripheral blood mononuclear cells derived from CD3⁺ cells. b Bubble chart representing receptor expression of CCR2, CCR3, CCR5 and CCR6 on CD4⁺ and CD8⁺ T cells. Size of the bubble corresponds to percentage-positive cells while colour represents the median fluorescence intensity (MFI). c CCR2 receptor expression on CD4⁺ and CD8⁺ T cells (% cells and MFI). d CCR6 receptor expression on CD4⁺ and CD8⁺ T cells (% cells and MFI). e t-SNE plots showing receptor expression distribution on CD4⁺ and CD8⁺ T cells. f Co-receptor expression of CCR2, CCR3, CCR5 and CCR6 on CD4⁺ T cells. g Co-receptor expression of CCR2, CCR3, CCR5, and CCR6 on CD8⁺ T cells. c, d Contour plots show a representing sample from PLWH_EC, PLWH_ART and HC corresponding to the median % of cells within each group. a, c, d, f, g Statistical significance was determined using two-tailed Mann–Whitney U-test (significance level p < 0.05, with *<0.05, **<0.001) and represented as pie charts or with median using 95% CI.