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. 2022 Apr 13;13(4):341. doi: 10.1038/s41419-022-04806-9

Fig. 1. OPN-induced ROS production promotes the cell proliferation and migration of HCC cells.

Fig. 1

The results of oxidoreductase activity and cell redox homeostasis in the tumor tissues of HCC patients (data from GEO data-set GSE76427) (n = 115) (A). The level of ROS in SMMC7721 cells after treatment with different concentrations of OPN recombinant protein (10 ng/mL, 100 ng/mL, 1 μg/mL) (B). CCK8 and transwell assays were performed to detect the viability and migration of SMMC7721 cells (C, E). The effects on cell viability and migration were analyzed in hOPN treated SMMC7721 cells after treating them with NAC (5 mM) for 2 h (D, F). The level of ROS in HCCLM3 cells, 72 h after infecting them with siOPN (G). The transwell assay and growth curve were performed to detect the proliferation and migration of HCCLM3 cells (H, I). Original magnification, ×100 (scale bars: 100 μm). Data indicated mean ± SD. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, # Compared with hOPN treatment.