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. 2022 Apr 13;13(4):341. doi: 10.1038/s41419-022-04806-9

Fig. 2. NOX1 is upregulated by OPN in HCC cells.

Fig. 2

qRT-PCR was performed to examine the effect of hOPN treatment (1 μg/mL) and OPN knockdown on the mRNA levels of NOX family (A, B). Western blot was performed to examine the effect of hOPN treatment (10 ng/mL, 100 ng/mL, 1 μg/mL) and OPN knockdown on the level of NOX1 expression (C, D). Immunohistochemical analysis of 24 human HCC samples stained with OPN and NOX1 antibodies (E). Original magnification, ×200 (scale bar: 50 μm). The correlation between the IHC scores of OPN and NOX1 was analyzed (F). Data indicated mean ± SD. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, NS not significant.