Skip to main content
. 2022 Apr 13;13(4):341. doi: 10.1038/s41419-022-04806-9

Fig. 6. NOX1-induced ROS stimulates JAK2/STAT3 pathway and amplifies the effect of JAK2/STAT3 by OPN in HCCLM3 cells.

Fig. 6

HCCLM3 cells were transfected with siNOX1 or negative control. A Western blot analysis of NOX1, pJAK2, and pSTAT3 expressions was performed after transfection. The levels of NOX1, pJAK2, and pSTAT3 proteins were analyzed after transfection with NOX1 overexpression plasmid or vector control (B). The levels of pJAK2 and pSTAT3 proteins were analyzed in NOX1 overexpressed HCCLM3 cells and NAC treated NOX1 overexpressed HCCLM3 cells (C). Western blot analysis was carried out for the levels of pJAK2 and pSTAT3 proteins of control HCCLM3 cell, hOPN treated HCCLM3 cells, siNOX1 HCCLM3 cells, and siNOX1 HCCLM3 cells treated with hOPN (D). Data indicated mean ± SD. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, # Compared with NOX1 overexpression or hOPN treatment.