Table 1.

Summary of donor DNA design and editing outcomes in mammalian embryos.

Donor DNA	5′ homology arm	3′ homology arm	Major editing type
			Point mutation	Insertion	Conditional allele
ssODN	≥30 nt	≥30 nt	OK	≤100 nt	Not efficient
lssDNA	≥55 nt	≥55 nt	OK	≤1,368 nt	OK
AAV	≥475 nt	≥475 nt	OK	≤3,300 nt	Unknown
Supercoiled plasmida	≥500 bp	≥500 bp	Unnecessary	≤7,100 bp	OK
Supercoiled plasmidb	800 bp	800 bp	Unnecessary	~800 bp	Unknown
Supercoiled plasmidc	40 bp	40 bp	Unnecessary	≤5,000 bp	OK
Linear plasmida	≥500 bp	≥500 bp	Unnecessary	≤7,100 bp	OK
Linear dsDNAd	800 bp	800 bp	Unnecessary	≤6,000 bp	Unknown

Note: It is unnecessary to used plasmids and linear dsDNA to generate point mutation mammals. a, HR. b, HMEJ. c, PITCh. d, Tild