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. 2022 Apr 13;5(7):e202101268. doi: 10.26508/lsa.202101268

Figure 1. iNGN–astrocyte co-culture system on transparent multi-electrode arrays (MEAs).

Figure 1.

(A) Experimental scheme of iNGN–astrocyte co-culture preparation (left) and experimental procedure (right) over time. Functional recordings, microscopy imaging, full-field, and holographic stimulations were studied on different days post induction (dpi). (B) Schematic of the so-called Banker culture, a neuron–glia co-culture system within the MEA device. (C) Representative microscopy images of iNGN cells. Upper panel, iNGN cells grown on Matrigel-coated well plates at 3 dpi. Lower panel, PDL-laminin–coated MEA chambers at 15 dpi. ChR2-EYFP–labeled cells are visualized by live cell microscopy. Scale bars, 100 μm. mTeSR1: standardized human induced pluripotent stem cell medium. BrainPhys: serum-free neurophysiological basal medium for improved neuronal function. plv-ef1α-ChR2-EYFP: lentiviral particles delivering ChR2-EYFP under the ef1α promotor to iNGN cells. Dox: doxycycline. Ara-C: cytosine arabinoside to remove undifferentiated cells.