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. 2022 Apr 13;5(7):e202101268. doi: 10.26508/lsa.202101268

Figure S11. Optical setups for full-field and holographic stimulations.

Figure S11.

For full-field stimulation light from a light-emitting diode array (470 nm) of a spectra 4 light source is coupled to a liquid waveguide (WG) and outcoupled to an inverted Nikon microscope. Light is directed onto the sample (S) by the tube lens L4 and a microscope objective (O). For holographic stimulation, the laser (450-nm laser diode) is collimated by L1 and directed orthogonally onto the spatial light modulator by the beam splitter. Modulated light is filtered in polarization to achieve a phase shift of pi and then de-magnified onto the sample by telescope L2-L3. L1 to L3, lenses; SLM, ferroelectric liquid-crystal spatial-light modulator; BS, Non-polarizing 50:50 beam splitter; LP, linear polarizer; S, sample.