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. 2022 Mar 31;10:867877. doi: 10.3389/fbioe.2022.867877

FIGURE 4.

FIGURE 4

Scratch assay performed on hTEMs seeded with ECFCs and cultured with FBS as medium supplement. Scratch was performed in the middle of the hTEM (red lines). ECFCs were cultured for up to 5 days under static (A–D) or dynamic orbital conditions (E–H), and finally stained for Collagen III (green), phalloidin (red) and DAPI (blue) at four different time points (0 h, 8 h, 2 days, and 5 days). (A,E): Control scratch performed at time 0, under static and dynamic orbital conditions, respectively. (B,F): ECFCs migration at 8 h timepoint under static and dynamic orbital conditions, respectively. (C,G): ECFCs migration at 2 days timepoint under static and dynamic orbital conditions, respectively. (D,H): ECFCs reached confluency after 5 days from scratch. (I–L): Schematic representation of the scratch assay test on hTEMs (Images adapted from Servier Medical Art under a creative common attribution 3.0 unported license) (500 µm scale bars).