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. 2022 Mar 31;10:866800. doi: 10.3389/fcell.2022.866800

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Copyright © 2022 Gupta, Ghosh, Chakravarti, Singh, Ravichandiran, Swarnakar and Ghosh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of Generalized structure of Cas13d. The Cas13d is also a bilobed effector protein consisting of one crRNA “Recognition” lobe (REC) and one “Nuclease” lobe (NUC). The REC lobe consists of two domains: the N-terminal domain (NTD) and a Helical-1 domain and the NUC lobe contain 3 domains: two HEPN (HEPN1 and HEPN2) domains and one Helical-2 domain. The REC lobe mainly functions in recognizing and interacting with the stem duplex crRNA repeat and a spacer region of crRNA while the NUC lobe primarily functions in stabilizing the binding of Cas13d-crRNA-target RNA ternary complex and carrying out nucleolytic cleavage of targeted ssRNA.