TABLE 1.
Summary of methods and criteria used to evaluate the MG-derived neurogenesis in adult mouse retina.
| Treatment | Gene manipulation | Lineage tracing | Intermediate status capture | Suggested mechanism |
|---|---|---|---|---|
| NMDA injury + Ascl1 OE + TSA | Mouse genetics (tetO-Ascl1-ires-GFP) | Stringent genetic-based (Glast-CreER;LNL-tTA or Rlbp1-CreER;LNL-tTA) | Genetic-based scRNA-seq (FACS of Ascl1-GFP+ cells) | Transdifferentiation; Two-step reprogramming? |
| NMDA injury + Nfi knockout + GF | Mouse genetics (Nfia/b/xlox/lox ) | Stringent genetic-based (Glast-CreER;CAG-LSL-Sun1-GFP) | Genetic-based scRNA-seq (FACS of Sun1-GFP+ cells) | Transdifferentiation; Two-step reprogramming? |
| No injury needed: 1, β-catenin OE 2, Otx2, Crx, Nrl | recombinant AAVs (GFAP-β-catenin) (GFAP-Otx2, Crx, Nrl) | Stringent genetic-based (GFAP-Cre;Rosa26-tdTomato) | Morphological visualization (AAV-GFAP-GFP and Rhodopsin-tdTomato) | Two-step reprogramming |
| No injury needed: Ptbp1 downregulation | recombinant AAVs (GFAP-CasRx-Ptbp1) | Non-stringent genetic-based (AAV-Cre in Ai9 reporter mice) | No | Transdifferentiation |
| No injury needed: Math5/Brn3b OE | recomninant AAVs (GFAP-Math5-Brn3b) | Non-stringent genetic-based (AAV-based GFP reporter in WT or Glast-CreER mice) | AAV-based scRNA-seq and morphological visualization (GFAP-Math5-Brn3b-GFP) | Transdifferentiation |
OE: overexpression; GF: Growth factors.