Figure 2.

L-F001 reduces RSL3-induced ROS production and lipid peroxidation in HT22 cells. (A) HT22 cells were treated with 0.1 μM RSL3 alone, and then added L-F001 (1–10 μM) and LA (10 μM) at 37°C for 24 h. ROS formation was measured with a fluorescence microscope (scale bar = 50 μm). (B) ROS levels were measured with a fluorescence plate reader (n = 3). (C) The lipid ROS formation was monitored by a laser confocal microscope using a C11-BODIPY581/591 probe (Scale bar = 25 μm). ^#P < 0.05 compared with the control group. *P < 0.05, **P < 0.01 compared with the RSL3-induced group.