Figure 4.

L-F001 reduces RSL3-induced impairment of iron homeostasis in HT22 cells. HT22 cells were treated with 0.1 μM RSL3 alone, and then added L-F001(10 μM) at 37°C for 24 h. (A) Intracellular Fe²⁺ level in HT22 cells were detected by FerroOrange probe (scale bar = 25 μm). The fluorescence intensity of the CT group was defined as 100% (n = 5). (B) Endogenous hydroxyl radicals’ level in HT22 cells were detected by Rho-Bob probe (Scale bar = 25 μm). (C) Protein level and analysis of FTH1 in cells (n = 3). ^##P < 0.01 and ^###P < 0.001 compared with the control group. **P < 0.01 compared with the RSL3-induced group.