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Effects of N-glycosylation on location and degradation pathway of TIM-4. (A) Confocal immunofluorescence microscopy was used to detect location of TIM-4 in TIM-4-transfected and N291Q-transfected A549 cells. (B) Confocal immunofluorescence microscopy was used to detect colocalization between TIM-4 and ER in TIM-4-transfected and N291Q-transfected A549 cells. (Scale bar:10μm). (C) Western Blot was used to detect the degradation pathway of TIM-4.
