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Real-time two-photon microscopy imaging of iWAT of GCaMP6f transgenic mice after 1 mg/kg NE stimulation. Ca2+ signal was obtained by using a fluorescent filter (Excitation 410–455 nm, Dichroic FT475, and Emission 495–540 nm). Videos were obtained for 8 min at 512 × 512 resolution with a sampling interval of 10 s. Frames were taken every 10 s for 8 min.
