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Real-time imaging of white adipocytes isolated from iWAT of GCaMP6f transgenic mice after 1 μM NE stimulation. Images were acquired every 1 s using a Zeiss LSM880 confocal microscope. The following filter Spectra was used: Excitation 470/40, Dichroic FT495, and Emission 525/50. For videos, the image sequences were saved as movies by Zeiss LSM880 (Applied Precision), and the aggregates move 30 times faster than in real time. Frames were taken every 1 s for 5 min.
