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. 2022 Apr 13;23:303. doi: 10.1186/s12864-022-08543-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Comparison of each expressed gene between TruSeq, SMARTer, and TeloPrime methods. A Violin plot of expressed genes. B Heat map of hierarchical clustering of 129 differential expressed genes (FDR < 0.05) between two samples using TruSeq, SMARTer, and TeloPrime. C A scatterplot [log2 (TPM + 1)] of total genes (26,475 genes). Red spots indicate the differential expression of genes (p < 0.05). D Boxplot of exon number in each cluster (129 differential expressed genes). E Boxplot of transcription length in each cluster (129 differential expressed genes)