TABLE 1.
Free sterols present in T. cruzi epimastigotes (EP stock) grown in the absence or presence of UR-9825 or ketoconazolea
| Name | Structure | Control | UR-9825
|
Ketoconazole (1 μM) | ||
|---|---|---|---|---|---|---|
| 0.01 μM | 0.03 μM | 1 μM | ||||
| Exogenous: cholesterol |  |
30.2 | 37.9 | 12.5 | 10.1 | 9.4 |
| Endogenous, 14-desmethyl 24-Methylcholesta-5,7,22-trien-3β-ol (ergosterol) | 36.9 | 14.2 | 5.2 | <1 | <1 | |
| 24-Ethylcholesta-5,7,22-trien-3β-ol | 15.4 | 22.1 | <1 | <1 | <1 | |
| Ergosta-5,7-dien-3β-ol | 6.2 | 9.7 | <1 | <1 | <1 | |
| Ergosta-5,7,24(241)-trien-3β-ol | 6.0 | <1 | <1 | <1 | <1 | |
| Ergosta-7,24(241)-dien-3β-ol | 5.3 | <1 | <1 | <1 | <1 | |
| 24-Ethyl-5,7-cholesta-dien-3β-ol | <1 | 4.0 | <1 | <1 | <1 | |
| Endogenous, 14-methyl 24-Methylenedihydrolanosterol | 2.2 | 47.0 | 62.5 | 61.9 | ||
| 4,14-Dimethylergosta-8,24(241)-dien-3β-ol (obtusifoliol) | <1 | <1 | 19.0 | 7.8 | 10.1 | |
| Lanosterol | <1 | 9.9 | 16.4 | 19.6 | 18.6 | |
a
Sterols were extracted from T. cruzi epimastigotes cultured in LIT medium in the absence or in the presence of the indicated concentrations of UR-9825 or ketoconazole for 120 h; they were separated from polar lipids by silicic acid column chromatography and were analyzed by quantitative capillary gas-liquid chromatography and mass spectrometry as described in Materials and Methods. Results are expressed as mass percent.