TABLE 2.

Phospholipid composition of T. cruzi epimastigotes (EP stock) grown in the absence or presence of UR-9825 or ketoconazole for 120 h^a

Phospholipid species	Control (no drug)	UR-9825		Ketoconazole (1 μM)
		0.03 μM	1 μM
Phosphatidylcholine	15.2 ± 1.8	13.7 ± 1.1	13.1 ± 0.8	11.7 ± 1.7
Phosphatidylethanolamine	23.9 ± 2.5	31.0 ± 2.8b	33.8 ± 2.8b	34.6 ± 2.9b
Phosphatidylinositol	13.4 ± 1.9	12.8 ± 2.5	17.2 ± 2.1	14.2 ± 2.8
Phosphatidylserine	31.4 ± 2.9	28.4 ± 2.1	26.8 ± 3.0	28.2 ± 2.8
Sphingomyelin	16.1 ± 1.2	14.1 ± 1.2	9.1 ± 1.5	11.3 ± 1.9

^aEpimastigotes were grown in LIT medium with and without the indicated drug concentrations for 120 h, and polar lipids were separated from the total lipid extract by silicic acid column chromatography and thin-layer chromatography as described in Materials and Methods. The different phospholipid species present were quantitated by measuring organic phosphorous. The results are expressed as mole percent and the means and standard errors are based on at least three independent experiments. 

^bP < 0.05 versus controls.