FIGURE 5.

High BCL2 interacting protein 3 (BNIP3) protein expression is associated with a low inflammatory profile in aged human muscle. (a) Quantification of BNIP3 protein expression in muscle biopsies from young (n = 23) and old (n = 99) human subjects. (b) Distribution of values for BNIP3 expression in young and old subjects. (c) Quantification of BNIP3 protein levels in young subjects (n = 23) and old subjects with the lowest BNIP3 expression (Old BNIP3^Low, n = 12) and the highest BNIP3 protein expression (Old BNIP3^High, n = 12). (d–f) Expression levels of the inflammatory markers NLRP3 (nucleotide‐binding oligomerization domain (NOD)‐, leucine‐rich repeat (LRR)‐, and pyrin domain‐containing protein 3) and cleaved caspase 1, and the denervation marker neural cell adhesion molecule (NCAM), in young, Old BNIP3^Low and Old BNIP3^High subjects. (g) Correlation between Charlson comorbidity index (CCI) and muscle BNIP3 protein expression levels in old subjects (n = 50). (H) CCI values in young, old BNIP3^Low, and old BNIP3^High subjects. Data are expressed as box and whiskers showing minimum to maximum data