Figure 7.

A potential cooperative pro-tumor effect of SPP1 and NETs. (A) EMT score differences between the NET score top—SPP1 top subset and the NET score bottom—SPP1 bottom subset for each tumor type. (B, C) Western blot (B) and qPCR (C) results showing successful siRNA knockdown of SPP1 in MHCC97H cells. (D, E) Transwell assay measuring the migration ability of MHCC97H cells with/without NET stimulation and with/without SPP1 knockdown. Scale bar:50 µm. (F) Exogenesis treatment with SPP1 protein (10 ng/ml) promotes neutrophil chemotaxis (upper) and NET formation. (G, H) Representative immunofluorescent image showing NET formation in an orthotopic HCC mouse model using Hepa1-6 cells with (H) or without (G) Spp1 overexpression. Scale bar: 50 µm. ns, P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.