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Knockout of AwAreA in WT, ΔAwku70, and ΔAwlig4. PCR identification of ΔAwAreA generated from WT (A), ΔAwku70 (B), and ΔAwlig4 (C). For each parental strain, a total of 23 transformants (2–24) were randomly selected for PCR identification, with a parental strain (1) as control. The copy number hygR cassettes for WT (D), ΔAwku70 (E), and ΔAwlig4 (F) were determined by real-time genomic PCR analyses.
