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. 2022 Mar 9;21(4):e13580. doi: 10.1111/acel.13580

FIGURE 3.

FIGURE 3

Role of the fibrogenic and the inflammatory SASPs in the regulation of myofibroblast phenotype. (a, b) qPCR analysis of the indicated genes in FF292 ER:RAS fibroblasts expressing inducible NICD (NICDi) or empty vector (V) treated with doxycycline in growing (EtOH) or senescence (4‐OHT) conditions. (c) Western blot analysis of α‐SMA (short and long exposures) and IL‐8 in the same conditions as in a and b. The mean and standard deviation from two independent infections are shown. (d, e) qPCR analysis of the indicated genes in FF292 ER:RAS fibroblasts expressing IκBα‐mut or empty vector (V). (f) Western blot analysis of α‐SMA and IL‐8 in the same cells as in d, e. The means and standard deviation from three independent experiments are shown. In a, the # symbol indicates significance relative to 4‐OHT/no Doxy/no NICDi; in e, the # symbol indicates significance relative to IκBα‐mut/EtOH