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. 2022 Mar 31;15:857216. doi: 10.3389/fnmol.2022.857216

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Copyright © 2022 Liu and Rask-Andersen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The human outer sulcus epithelium demonstrates many red puncta representing GJB6 transcripts. These are also seen in type II fibrocytes. Inset shows root cells at higher magnification. (B) ATP1B1 gene transcripts are also expressed in the outer sulcus epithelium and in type II fibrocytes. Inset shows a type II fibrocyte at higher magnification with mRNA transcripts located in the nucleus (Nu) and at nuclear pore regions. (C) SR-SIM shows expression of Cx26 and Cx30 in the root cells. Inset (D) shows a root cell expressing ATP1B1. Framed area is magnified in (E), showing several large Cx30 GJ plaques in the root cells and associated Cx26 plaques (arrows). OS, outer sulcus; Cap, capillary.