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. 2022 Mar 31;15:857216. doi: 10.3389/fnmol.2022.857216

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Copyright © 2022 Liu and Rask-Andersen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Multiplex RNAscope^® ISH and SR-SIM of the Hensen cell region. Cell nuclei contain many ATP1A1, ATP1B1, and GJB6 gene transcripts. Framed area is magnified in (B). (B) The 1cell nucleus contains all three transcripts, suggesting active transcription of NKA and Cx30. (C) SR-SIM of Cx30-positive GJ plaques (green) are seen between Hensen cells. (D) Framed area in (C) is magnified. (E) Confocal microscopy showed expression of NKAβ1 in the lateral cell membranes of Hensen cells. Cx30 was also expressed.